Process of Recombinant DNA Technology

Choose the option that is showing the correct sequence of events occurring in each cycle of polymerase chain reaction (PCR).

Which of the following technique is used for separating the fragments of DNA cut by restriction endonucleases?

The source organisms that possess Taq polymerase are

As opposed to DNA replication within the cell, discontinuous synthesis of DNA does NOT occur in a polymerase chain reaction (PCR). Why?

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

A. Insertion of recombinant DNA into the host cell.

B. Cutting of DNA at specific location by restriction enzyme.

C. Isolation of desired DNA fragment.

D. Amplification of gene of interest using PCR.

Choose the correct answer from the options given below: 

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

Upon exposure to UV radation, DNA stained with ethidium bromide will show:

Small chemically synthesised oligonucleotides that are complementary to the regions of DNA are called primers and present at the

Given below are two statements about polymerase chain reactions.  

P) It mimics DNA replication that happens in a cell.

Q) It cannot be used to amplify RNA molecules.  

Gel electrophoresis is used for

The first recombinant DNA was constructed using plasmid of the following bacterium

In gel electrophoresis, separated DNA fragments can be visualized with the help of

In a PRC reaction, only the reverse primer was omitted from the reaction mixture. How many single stranded (ss) and double stranded (ds) DNA molecules, excluding primer molecules, will be present in the mixture at the end of 5 cycles, given 10 molecules of double stranded DNA were used as template?

In recombinant DNA technology, which dye is used to stain the separated DNA fragments which can be visualised by exposure to UV radiation.

Choose the correct option that represents correct sequential steps for PCR method:

Complete the analogy and select the correct option. 

$\mathrm{Zinc} \mathrm{ion} : \mathrm{Enzyme} \mathrm{activation} ::\mathrm{Calcium} \mathrm{ion} : \mathrm{\_\_\_\_\_}$.